RIDASCREEN® Spezifisches IgG
Product Code A0629
Intended use:
For in vitro diagnostic use. The RIDASCREEN® Spezifisches IgG test is an enzyme immunoassay (EIA) for the quantitative determination of specific IgG antibodies in human serum and should be used in cases of suspected Type III allergy. However, the test can also be used for the follow up of hyposensitisation to hymenoptera toxins etc. This test is not suitable for HSA allergens and multi discs.
General information:
Type III allergy and its trigger can be determined by the detection of
specific IgG antibodies. Type III allergies are characterised by the
formation of immune complexes formed from free allergens and the
specific IgG antibodies. These immune complexes can also be formed by
external allergens on body surfaces such as the lungs, and lead to the
clinical picture of exogenetic allergic alveolitis. Farmer’s lung or
pigeon breeder’s disease is caused by constant exposure to fungus from
mouldy hay or pigeon antigens.
These immune complexes can also develop as a result of food or
food components penetrating through the wall of the intestine,
depositing in the tissue and causing inflammation reactions, thus
leading to a variety of clinical pictures.
The determination of IgG antibodies is also used for therapeutic monitoring in the case of allergies to hymenoptera toxins.
The test is a cellulose-disc-based enzyme allergo-sorbent test
(EAST). All reagents (A0021) listed under Section 4 are validated using
the allergen discs (A0029) from R-Biopharm. When using these reagents
with discs from other suppliers, it is the duty of the user to carry
out a validation test.
Test principle
The allergens are attached to the cellulose discs. In order to
carry out the test, the allergen discs are placed in the wells of a
microwell plate or are pre-coated according to the requirements of the
doctor. Patient sera, standard sera and negative and positive controls
are pipetted onto the allergen discs and incubated at 37°C. During
incubation, the allergy-specific IgG antibodies attach themselves to
the allergen. Material which does not attach is removed by washing.
After this, an anti-human IgG antibody, which is conjugated with
alkaline phosphatase, is also added. Any conjugate which is not
attached is removed by washing. Substrate is then added and is
dephosphorylated by the conjugated enzyme into a yellow product. The
intensity of the yellow colour is proportional to the quantity of
allergen-specific IgG antibodies in the serum.
A photometric measurement is carried out at 405 nm using a reference wavelength of 620 nm.
Specifications:
Standards: | 2,5 µg/ml - 200 µg/ml |
Incubation time: | 150 min |