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Allergy Diagnostics

  • Absolutely essential for allergy diagnostics
  • Enzyme immunoassay, immunoblot

RIDASCREEN® Spezifisches IgG

Product Code A0629

Intended use:

For in vitro diagnostic use. The RIDASCREEN® Spezifisches IgG test is an enzyme immunoassay (EIA) for the quantitative determination of specific IgG antibodies in human serum and should be used in cases of suspected Type III allergy. However, the test can also be used for the follow up of hyposensitisation to hymenoptera toxins etc. This test is not suitable for HSA allergens and multi discs.

General information:

Type III allergy and its trigger can be determined by the detection of specific IgG antibodies. Type III allergies are characterised by the formation of immune complexes formed from free allergens and the specific IgG antibodies. These immune complexes can also be formed by external allergens on body surfaces such as the lungs, and lead to the clinical picture of exogenetic allergic alveolitis. Farmer’s lung or pigeon breeder’s disease is caused by constant exposure to fungus from mouldy hay or pigeon antigens.
These immune complexes can also develop as a result of food or food components penetrating through the wall of the intestine, depositing in the tissue and causing inflammation reactions, thus leading to a variety of clinical pictures.
The determination of IgG antibodies is also used for therapeutic monitoring in the case of allergies to hymenoptera toxins.
The test is a cellulose-disc-based enzyme allergo-sorbent test (EAST). All reagents (A0021) listed under Section 4 are validated using the allergen discs (A0029) from R-Biopharm. When using these reagents with discs from other suppliers, it is the duty of the user to carry out a validation test.

Test principle
The allergens are attached to the cellulose discs. In order to carry out the test, the allergen discs are placed in the wells of a microwell plate or are pre-coated according to the requirements of the doctor. Patient sera, standard sera and negative and positive controls are pipetted onto the allergen discs and incubated at 37°C. During incubation, the allergy-specific IgG antibodies attach themselves to the allergen. Material which does not attach is removed by washing. After this, an anti-human IgG antibody, which is conjugated with alkaline phosphatase, is also added. Any conjugate which is not attached is removed by washing. Substrate is then added and is dephosphorylated by the conjugated enzyme into a yellow product. The intensity of the yellow colour is proportional to the quantity of allergen-specific IgG antibodies in the serum.
A photometric measurement is carried out at 405 nm using a reference wavelength of 620 nm.

Specifications:

Standards: 2,5 µg/ml - 200 µg/ml
Incubation time: 150 min
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