RIDASCREEN® Bordetella IgA

Product Code. K2511

Intended use:

For in vitro diagnostic use. The RIDASCREEN^® Bordetella IgA test is an enzyme immunoassay for the semi-quantitative determination of IgA antibodies against Bordetella pertussis in human serum. The test should be used for confirmation purposes when there is a suspected case of infection with Bordetella pertussis or for clarifying the immune status.

 General information:

Bordetella pertussis is the causative agent of whooping cough. The incubation period takes 7 - 21 days. Specific antibodies are normally detectable with the beginning of the clinical symptoms. Despite an infection or vaccination during childhood, an immunity for life is not generated. Adults with first or second time infection do normally not show the severe picture of whooping cough. However, they represent a source of infection for others. With the ELISA technology, a sensitive method for antibody detection is available with the possibility to differentiate between the individual immunoglobulin classes. This is especially important for the differentiation between a natural infection and an antibody titer due to a vaccination. Specific IgG and IgM are increased following infection or vaccination; whereas, specific IgA is increased only following infection.

Test principle
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second incubation step by using enzyme-labelled anti-human antibodies (the conjugate). The enzyme converts the colourless substrate (H₂O₂/TMB) to a blue end product. The enzyme reaction is stopped by adding sulphuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength ≥ 620 nm.

Specifications: