RIDASCREEN® Tetanus IgG
Product Code K3721
Intended use:
The RIDASCREEN® Tetanus test is a quantitative enzyme immunoassay (EIA) for specific detection of IgG antibodies against the tetanus toxoid in human serum. The test should be used for determination of the immune status before or after vaccination.
General information:
Tetanus (lockjaw) is caused by a toxin produced by Clostridium tetani.
Clostridium tetani is an ubiquitous bacterium in soils. After open
wounds have become infected, the pathogen penetrates into the tissue
where it multiplies anaerobically excreting the toxin.
Because immunization is carried out in infancy using a toxoid
vaccine, the risk of infection is low in industrialized countries. But,
vaccination has to be repeated every 5 - 10 years. Injured people with
vaccine protection should receive a booster as a precaution if their
last vaccination is more than five years ago. Human antitoxin must be
administered when the vaccination status is not clear.
The vaccination status can be determined using a modern EIA. The
comparability of measured values is ensured by using a toxoid antigen
and matching to international units. Nevertheless, it has to be
considered for EIA result validation that both the extent of the immune
reaction after an vaccination and the period of vaccine protection may
vary from patient to patient.
Test principle
Purified antigens are coated to a microwell plate. Antibodies in
the patient samples bind to the antigens and are determined during the
second incubation step by using enzyme-labelled anti-human antibodies
(the conjugate). The enzyme converts the colourless substrate (H2O2/TMB)
to a blue end product. The enzyme reaction is stopped by adding
sulphuric acid and the colour of the mixture switches from blue to
yellow at the same time. The final measurement is carried out at 450 nm
on a photometer using a reference wavelength ≥ 620 nm.
Specifications:
Format: | Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells) |
Incubation time: | 3 x 30 min at 37 ºC |
Sensitivity: | 92.9% |
Specificity: | 100.0% |