EIAgen Total T4

brief description

It has molecular weight of 777 daltons and is synthesized by iodination of tyrosine residues on thyroglobulin. This is stored in the follicles of the thyroid gland and T4 is released into the circulation following enzymatic cleavage from thyroglobulin. Both the formation and the release of T4 are promoted by thyroid stimulating hormone (TSH), produced by the pituitary. T4 itself inhibits the release of TSH, so completing a classical negative feedback loop. life of 6 to 7days and is strongly but reversibly bound to serum proteins, mainly thyroxine binding globulin (TBG). Only a small fraction (approximately 0.015%) is present in the circulation in the free or unbound form. It is thought that the free fraction exerts the main influence on metabolic control.

intended use

EIAgen Total T4 is a direct solid phase enzyme immunoassay for the quantitative determination of total Thyroxine (T4) in human serum or plasma.