RIDASCREEN® HSV 1+2 IgG
Product Code K5021
Intended use:
For in vitro diagnostic use. The RIDASCREEN® HSV 1+2 IgG test is an enzyme immunoassay (EIA) for the semi-quantitative determination of IgG antibodies against Types 1 and 2 of the Herpes simplex virus (HSV 1 and HSV 2) in human serum. The test is based on total antigen and reacts with antibodies against both types in parallel. It should be used for screening for IgG antibodies against HSV.
General information:
After infection with HSV, specific antibodies are formed against the
pathogen as a result of the response from the immune system. By using
immunological methods, it is possible to determine the antibodies in
the serum. The test method used and the choice of the pathogen-specific
antigen both have a significant bearing on the meaningfulness of the
test.
Because it is possible to differentiate between the individual
immunoglobulin classes in the enzyme immunoassay, more precise
statements about the immunological status of a patient can be made than
those which are based on other serological methods (such as the
haemagglutination inhibition test or the complement fixation test).
Test principle
Purified antigens are coated to a microwell plate. Antibodies in
the patient samples bind to the antigens and are determined during the
second incubation step by using enzyme-labelled anti-human antibodies
(the conjugate). The enzyme converts the colourless substrate (H2O2/TMB)
to a blue end product. The enzyme reaction is stopped by adding
sulphuric acid and the colour of the mixture switches from blue to
yellow at the same time. The final measurement is carried out at 450 nm
on a photometer using a reference wavelength ≥ 620 nm.
Specifications:
Format: | Microtiter plate with 96 wells (12 strips with 8 wells each; strips can be divided into single wells) |
Incubation time: | 3 x 30 min at 37 °C |
Sensitivity: | 97.8% |
Specificity: | 100.0% |